Production of Influenza H5 Vaccine Oligomers in Plants

The transient expression of veterinary vaccines in plants is a promising tool because of its low cost connected with a practically unlimited scale-up. To achieve these goals, two major challenges, high immunogenicity of vaccines and minimal of down-stream processing cost, have to be overcome. Here we present and discuss protocols enabling to generate highly immunogenic H5 influenza candidate vaccines as H5 oligomers, by transient expression in Nicotiana benthamiana plants and to perform analytical experiments as Western blot, ELISA, and hemagglutination and hemagglutination inhibition assays.