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A Versatile Method of Patterning Proteins and Cells
Published on: February 26, 2017
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Surface patterning techniques for proteins on nano- and micro-systems: a modulated aspect in hierarchical structures.
1Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM'S NMIMS, V. L. Mehta Road, Vile Parle (W), Mumbai, India. shendepravin94@gmail.com.
Journal of Materials Chemistry. B
|February 4, 2022
Summary
Protein patterning techniques enable advanced biomedical applications. This review covers physical, physicochemical, and chemical methods for precise protein arrangement, crucial for bioengineering and diagnostics.
Area of Science:
- Biomedical Engineering
- Surface Science
- Nanotechnology
Background:
- Protein surface patterning is vital for bioengineering, biosensing, and antifouling applications.
- Existing methods involve fabrication and external functionalization of structures.
Purpose of the Study:
- To review advances in protein patterning technology.
- To emphasize physical and physicochemical methods for protein arrangement.
- To discuss chemical modification of surfaces and analytical evaluation methods.
Main Methods:
- Stencil patterning, microfluidics (trap- and droplet-based).
- Surface chemical modification via photolithography, microcontact printing, and scanning probe nanolithography.
- Robotic printing, stencil printing, colloidal lithography, and photochemical alterations.
Main Results:
- Physical confinement, electrostatic, capillary forces, and dielectrophoretics are key design concepts.
- Modified substrates include self-assembled monolayers on gold surfaces.
- Patterning techniques influence protein functionalization and orientation.
Conclusions:
- Protein patterning methods are selective and applicable for fabricating modified surfaces and target-specific delivery systems.
- Future applications include high-throughput screening, intracellular interaction studies, and personalized treatments.
