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Related Experiment Video

Updated: Oct 4, 2025

Single-cell Microfluidic Analysis of Bacillus subtilis

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

Investigating cell autonomy in microorganisms.

Sarah Piccirillo¹, Andrew P Morgan¹, Andy Y Leon¹

¹Department of Genetics, Developmental and Evolutionary Biology, School of Biological and Chemical Sciences, University of Missouri-Kansas City, 5100 Rockhill Rd., Kansas City, MO, 64110, USA.

Current Genetics

|February 4, 2022

Summary

This summary is machine-generated.

A new chimeric colony assay (CCA) detects cell-cell signaling in microbes. This genetic method reveals how genes affect neighboring cells, advancing our understanding of microbial communication.

Keywords:

Cell-nonautonomy Cell–cell signal Colony patterning Density dependence Escherichia coli Localization Saccharomyces cerevisiae

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Last Updated: Oct 4, 2025

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Cell-cell signaling in microorganisms is not well understood.
Existing methods for studying microbial communication are limited.

Purpose of the Study:

To introduce and validate the chimeric colony assay (CCA) for detecting cell-nonautonomous genetic effects.
To demonstrate the broad applicability of the CCA across different microbial systems.

Main Methods:

The chimeric colony assay (CCA) was developed to measure gene effects on neighboring cells.
The CCA was applied to study GAL1 transcription in Saccharomyces cerevisiae and translational readthrough in Escherichia coli.
Variations of the CCA and essential controls were described.

Main Results:

The CCA successfully detected cell-nonautonomous effects in both yeast and E. coli.
The assay demonstrated versatility in measuring various biological activities like transcript accumulation and protein localization.
The study validated the CCA's potential beyond its initial application in yeast colony patterning.

Conclusions:

The chimeric colony assay (CCA) is a powerful genetic tool for investigating cell-cell signaling in microbes.
The CCA offers a versatile platform for exploring microbial communication and gene function.
This method expands the scope of genetic assays for understanding microbial processes.