Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

8.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coil-Globule versus Field-Driven Coil-Blob Transitions of PEG under Nanopore Confinement.

ACS measurement science au·2026
Same author

Zero-Dalton Resolution in Nanopore Peptide Recognition.

Analytical chemistry·2026
Same author

Unlocking Rare 3-<i>O</i>-Sulfation Patterns in Heparan Sulfate via ARSG-Directed Regioselective Desulfation and HILIC-MS.

Analytical chemistry·2026
Same author

Profiling protein-binding glycosaminoglycan oligosaccharides using a simple label-free electrophoretic assay.

Glycobiology·2026
Same author

Structurally defined synthetic heparin oligosaccharides reveal unique signatures for nanopore structural analysis of GAGs.

Carbohydrate polymers·2025
Same author

Sequence and Force Effects on ssDNA Translocation through a Protein Nanopore: Coarse-Grained Steered Molecular Dynamics Simulations.

The journal of physical chemistry. B·2025
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Oct 4, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.0K

Nanopore-Based Protein Identification.

Mazdak Afshar Bakshloo1, John J Kasianowicz2,3, Manuela Pastoriza-Gallego1

  • 1CY Cergy Paris Université, CNRS, LAMBE, Cergy, 95000, France.

Journal of the American Chemical Society
|February 4, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a new nanopore method for identifying protein fragments. This rapid, simple technique uses aerolysin protein nanopores to detect single molecules, advancing proteomic analysis in cell biology and medicine.

More Related Videos

Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.7K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

Related Experiment Videos

Last Updated: Oct 4, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

14.0K
Monitoring Protein Adsorption with Solid-state Nanopores
08:51

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

13.7K
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.8K

Area of Science:

  • Biophysics
  • Proteomics
  • Analytical Chemistry

Background:

  • Current whole-proteome identification relies on protease digestion, mass spectrometry, and database matching.
  • Existing methods can be complex and time-consuming, limiting broad application in research and clinical settings.

Purpose of the Study:

  • To develop a reliable, rapid, inexpensive, and simple method for whole-proteome identification.
  • To demonstrate the utility of aerolysin nanopores for single-molecule protein fragment detection and classification.

Main Methods:

  • Utilized a nanometer-scale pore formed by the aerolysin protein.
  • Detected and classified polypeptide fragments generated by protease (trypsin) treatment of proteins.
  • Analyzed changes in ionic current through the nanopore caused by individual polypeptide fragments.

Main Results:

  • Successfully detected and classified polypeptide fragments from three different proteins treated with trypsin.
  • Fragment identification was achieved at the single-molecule level.
  • Nanopore-based fragment identification results were consistent with predicted trypsin cleavage patterns.

Conclusions:

  • Aerolysin nanopores offer a promising new approach for rapid and simple protein identification.
  • This single-molecule detection method has significant potential for advancing cell biology research and clinical diagnostics.
  • The technique provides an alternative to traditional mass spectrometry-based proteomic analysis.