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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: May 8, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Alternative 5' exons in c-abl mRNA.

Y Ben-Neriah, A Bernards, M Paskind

    Cell
    |February 28, 1986
    PubMed
    Summary
    This summary is machine-generated.

    The cellular abl proto-oncogene, a protein-tyrosine kinase, shows varied mRNA sizes due to alternative splicing. This splicing occurs at the same site involved in the Philadelphia chromosome translocation.

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    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    Related Experiment Videos

    Last Updated: May 8, 2026

    Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
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    Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
    09:58

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    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    Area of Science:

    • Molecular Biology
    • Oncogenesis
    • Gene Expression

    Background:

    • The cellular abl proto-oncogene encodes a protein-tyrosine kinase.
    • This gene is expressed in various cell types, typically as two or three distinct mRNA size variants.

    Purpose of the Study:

    • To investigate the structural diversity of mouse c-abl cDNAs.
    • To understand the mechanisms generating heterogeneity in c-abl mRNA.
    • To explore the relationship between c-abl splicing and oncogenic transformations.

    Main Methods:

    • Cloning of four types of mouse c-abl complementary DNAs (cDNAs) from 70Z/3 lymphoid cells.
    • Analysis of the 5' sequences of the cloned cDNAs to determine predicted N-terminal amino acid sequences.
    • Comparison of c-abl cDNA sequences with viral abl (v-abl) sequences.

    Main Results:

    • Four distinct mouse c-abl cDNAs were identified, each with unique 5' sequences encoding predicted N-terminal regions of 20-45 amino acids.
    • One cDNA shared a met-gly-gln N-terminal sequence with the gag N terminus of v-abl.
    • The observed 5' heterogeneity in c-abl cDNAs is attributed to the alternative addition of 5' exons to a common set of 3' exons.
    • Alternative splicing was found to occur at the identical site where BCR sequences attach to ABL sequences in the Philadelphia chromosome translocation.

    Conclusions:

    • The cellular abl proto-oncogene exhibits significant structural diversity at the mRNA level.
    • Alternative splicing is a key mechanism generating different forms of c-abl.
    • The splicing site involved in c-abl heterogeneity is conserved in the context of oncogenic translocations like the Philadelphia chromosome.