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Updated: Oct 4, 2025
Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper
Published on: February 27, 2021
Enzymatic modification of native chitin and chitin oligosaccharides by an alkaline chitin deacetylase from
Guang Yang1, Xiaoyue Hou1, Jing Lu1
1Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China; Jiangsu Marine Resources Development Research Institute, Jiangsu Ocean University, Lianyungang 222000, China; College of food science and engineering, Jiangsu Ocean University, Lianyungang 222005, China.
Abstract:
In this study, chitin deacetylase from Microbacterium esteraromaticum MCDA02 (MeCDA) was purified by ammonium sulfate precipitation, anion exchange chromatography, and superdex column chromatography. The molecular weight of purified MeCDA was approximately 26 kDa. The optimum pH and temperature of purified MeCDA were 8.0 and 30 °C, respectively. The enzyme activity is enhanced by metal ions K+ and Sr+ and inhibited by Co2+, Cd2+, and EDTA. The degree of deacetylation through enzymatic modification of MeCDA was removed an average of 32.75% of the acetyl groups for ɑ-chitin by acid-base titration. Meanwhile, MeCDA can catalyze the hydrolytic cleavage of the acetamido bond in GlcNAc units within chitin oligomers and polymers. Hence, the MeCDA is a potent chitin decomposer to catalyze chitin and chitin oligosaccharides deacetylation to prepare chitosan and chitosan oligosaccharide. This is a value-added utilization of chitin based biological resources.
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