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Updated: Oct 4, 2025
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Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses
Published on: January 17, 2025
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Anatomical variability, multi-modal coordinate systems, and precision targeting in the marmoset brain
Takayuki Ose1, Joonas A Autio2, Masahiro Ohno2
1Laboratory for Brain Connectomics Imaging, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan; Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
Neuroimage
|February 5, 2022
Summary
Accurate brain region targeting in marmosets requires accounting for individual anatomical differences. This study developed a neuroimaging pipeline to precisely locate brain areas, creating new templates for marmoset neuroscience research.
Area of Science:
- Neuroscience
- Primate Research
- Medical Imaging
Background:
- Standard stereotactic atlases lack precision due to individual anatomical variations in animal models.
- The common marmoset is a valuable model in systems neuroscience, necessitating refined targeting methods.
Purpose of the Study:
- To develop a non-invasive, multi-modal neuroimaging pipeline for precise brain region localization in common marmosets.
- To address intersubject anatomical variability in cranial and cortical landmarks for accurate targeting.
- To create novel multi-modal templates and atlases for marmoset brain research.
Main Methods:
- Developed a neuroimaging-based targeting pipeline using multi-modal data (head CT, brain MRI).
- Created population-based templates (MarmosetRIKEN20) in anterior-posterior commissure (AC-PC) and CIFTI grayordinate systems.
- Validated targeting accuracy using robot-guided surgery.
Main Results:
- Identified significant rotation (10 degrees) and bias in the stereotactic coordinate system relative to AC-PC.
- Demonstrated substantial variation in common cranial and brain landmarks across individual marmosets.
- Achieved high-precision targeting of deep brain structures (0.2 mm accuracy).
Conclusions:
- Subject-specific neuroimaging is crucial for precise targeting in marmoset brain studies due to significant anatomical variability.
- The developed pipeline and new templates (MarmosetRIKEN20) advance precision in marmoset neuroscience.
- These resources facilitate bridging macroscale and microscale analyses in marmoset research.
