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Bacterial identification and adhesive strength evaluation based on a mannose biosensor with dual-mode detection
Feiyun Cui1, Xiaoqun Shen1, Bo Cao2
1Key Disciplines Laboratory of Novel Micro-nano Devices and System Technology & Key Lab for Optoelectronic Technology and Systems, Ministry of Education, School of Optoelectronic Engineering, Chongqing University, Chongqing, 400044, China.
Biosensors & Bioelectronics
|February 5, 2022
Summary
A novel biosensor using mannose nano-surfaces can identify bacteria and measure their adhesive strength. This technology accurately distinguishes bacterial species and quantifies adhesion, aiding in microbial analysis.
Area of Science:
- Biomedical Engineering
- Microbiology
- Analytical Chemistry
Background:
- Bacterial adhesion is crucial for infections and biofilm formation.
- Accurate identification and quantification of bacterial adhesive strength are needed.
- Mannose is a key carbohydrate involved in bacterial adhesion mechanisms.
Purpose of the Study:
- To develop a biosensor for identifying bacteria and evaluating their adhesive strength.
- To utilize a mannose nano-surface for selective bacterial capture.
- To correlate electrochemical and spectroscopic data with bacterial adhesion.
Main Methods:
- Fabrication of a mannose nano-surface integrated biosensor.
- Application of Electrochemical Impedance Spectroscopy (EIS) for binding affinity measurements.
- Utilized Surface-Enhanced Raman Spectroscopy (SERS) coupled with Partial Least Squares Discriminant Analysis (PLS-DA) for bacterial identification.
Main Results:
- The biosensor successfully captured S. typhimurium and E. coli JM109.
- SERS-PLS-DA achieved 100% accuracy in discriminating between captured bacterial species.
- EIS measurements revealed higher binding affinity for S. typhimurium (6.859 × 10^23 M⁻¹) than E. coli (2.054 × 10^17 M⁻¹), indicating stronger adhesion.
Conclusions:
- The developed biosensor provides both qualitative and quantitative analysis of bacteria-mannose interactions.
- Normalized Impedance Change (NIC) serves as a reliable indicator of bacterial adhesive strength.
- This technology offers a promising tool for selective bacterial recognition and adhesion assessment.
