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Transcriptome analysis reveals long non-coding natural antisense transcripts involved in muscle development in fetal
Siyuan Zhan1, Yanan Xue1, Liu Yang1
1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, PR China; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, PR China.
Genomics
|February 6, 2022
Summary
This study identifies 466 antisense long non-coding RNAs (ASlncRNAs) in goat muscle, revealing their potential roles in embryonic muscle development through gene regulation and miRNA precursor production.
Area of Science:
- Genomics
- Molecular Biology
- Developmental Biology
Background:
- Non-coding RNAs are crucial for muscle development.
- The specific roles of antisense long non-coding RNAs (ASlncRNAs) in embryonic muscle development remain largely unexplored.
Purpose of the Study:
- To identify and characterize ASlncRNAs in goat longissimus dorsi muscle.
- To investigate the potential regulatory functions of ASlncRNAs in muscle development.
Main Methods:
- Bioinformatic identification of ASlncRNAs from muscle tissue.
- Differential expression analysis and quantitative PCR (qPCR) validation.
- Prediction of target genes and miRNA precursor analysis.
Main Results:
- Identified 466 ASlncRNAs, with 48 differentially expressed (P < 0.05).
- Predicted interactions between ASlncRNAs and 335 protein-coding genes, suggesting negative gene regulation.
- Identified 170 ASlncRNAs as potential miRNA precursors.
Conclusions:
- ASlncRNAs play a significant role in embryonic muscle development in goats.
- ASlncRNAs regulate muscle development by targeting protein-coding genes and acting as miRNA precursors.
- This catalog provides a foundation for further research into ASlncRNA function in muscle biology.
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