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Updated: Oct 4, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.1K

The role of splicing factor PRPF8 in breast cancer

Difei Cao1, Jiaying Xue1, Guoqing Huang1

  • 1Institute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin, Heilongjiang, China.

Technology and Health Care : Official Journal of the European Society for Engineering and Medicine
|February 6, 2022
PubMed
Abstract

Insights

High PRPF8 expression indicates poor prognosis in breast cancer, impacting survival and metastasis. This splicing factor influences key cancer pathways and cell growth, highlighting its role in disease progression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Alternative splicing generates protein diversity from single genes.
  • Splicing factors are implicated in cancer development.
  • PRPF8 is a crucial component of the spliceosome complex.

Purpose of the Study:

  • To investigate the prognostic significance of PRPF8 in cancer.
  • To explore the role of PRPF8 in breast cancer progression and survival.

Main Methods:

  • Analysis of PRPF8 expression across various cancer types.
  • Comparison of PRPF8 levels in breast cancer versus adjacent tissues.
  • Survival analysis, including distant metastasis-free survival (DMFS) and post-progression survival (PPS).
  • Gene Set Enrichment Analysis (GSEA) to identify associated pathways.
  • In vitro experiments involving PRPF8 silencing in MCF-7 cells.

Main Results:

  • PRPF8 is expressed in all tested cancer types.
  • Significantly different PRPF8 expression observed between breast cancer and paracancerous tissues.
  • High PRPF8 expression correlates with poor prognosis, reduced DMFS, and PPS.
  • GSEA revealed PRPF8 expression is linked to TGF-β, JAK-STAT, and cell cycle pathways.
  • PRPF8 silencing in MCF-7 cells reduced cell growth, colony formation, and increased p21 expression.

Conclusions:

  • PRPF8 is a significant prognostic factor in breast cancer.
  • PRPF8 plays a critical role in breast cancer progression.
  • Targeting PRPF8 may offer therapeutic potential for breast cancer.
Keywords:
PRPF8alternative splicingbreast cancerprognosis

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