Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microtubule dynamics in interphase cells.

E Schulze, M Kirschner

    The Journal of Cell Biology
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Researchers studied microtubule dynamics in vivo using labeled tubulin. They found rapid microtubule growth and turnover, with some stable microtubules potentially serving specialized functions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Normalized Raman Imaging for Studies of Tissue Physiology of the Kidney.

    bioRxiv : the preprint server for biology·2025
    Same author

    School-based physical activity interventions: which intervention characteristics are associated with participation and retention? A meta-analysis.

    Preventive medicine·2024
    Same author

    Ultrasound measurement technique for the single-turn-coil magnets.

    The Review of scientific instruments·2021
    Same author

    [Side effects of novel cancer immunotherapies].

    Der nephrologe·2020
    Same author

    Evaluation of a Recently Established Test for Familial Hyperaldosteronism Type 1.

    Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme·2016
    Same author

    Overnight switching from oxcarbazepine to eslicarbazepine acetate: an observational study.

    Acta neurologica Scandinavica·2016
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Area of Science:

    • Cell Biology
    • Cytoskeleton Dynamics
    • Microtubule Assembly

    Background:

    • Microtubules are essential cytoskeletal components involved in cell division, intracellular transport, and cell structure.
    • Understanding microtubule dynamics, including growth and turnover, is crucial for comprehending cellular processes.
    • In vivo studies are needed to validate in vitro models of microtubule steady-state dynamics.

    Purpose of the Study:

    • To investigate the sites and kinetics of microtubule growth in living cells (in vivo).
    • To determine the rate of tubulin subunit incorporation and microtubule turnover.
    • To compare in vivo microtubule dynamics with existing in vitro models.

    Main Methods:

    • Microinjection of biotin-labeled tubulin into living cells.

    Related Experiment Videos

  • Visualization using immunofluorescence and immunoelectron microscopy.
  • Analysis of subunit incorporation into existing microtubule structures.

    • Main Results:

    • Biotin-labeled tubulin subunits were incorporated into new microtubule growth segments, contiguous with unlabeled microtubules.
    • Microtubule elongation occurred through addition to existing microtubules and de novo nucleation at the centrosome.
    • Observed a rapid growth rate of 3.6 micron/min, independent of labeled tubulin concentration, leading to ~80% subunit exchange in 15 min.
    • Identified a population of quasi-stable microtubules with slower subunit exchange, suggesting specialized cellular roles.

    Conclusions:

    • In vivo microtubule dynamics are characterized by rapid elongation and turnover, with significant subunit exchange.
    • The observed kinetics challenge the direct applicability of some in vitro steady-state models to the in vivo situation.
    • Quasi-stable microtubules may represent a distinct functional class within the cell.