Related Experiment Video
Updated: Oct 4, 2025

Determining Viral Disinfection Efficacy of Hot Water Laundering
Published on: June 21, 2022
Effective disinfecting of protective devices used in the course of treating SARS-CoV2-positive patients
Moarie Grace Tan1, Tang Gui Feng2, Lim Teck Liang2
1Operating Theatre Services, Sengkang General Hospital, Singapore.
Background:
Powered Air-Purifying Respirator (PAPR) was widely used in Sengkang General Hospital (SKH) during the SARS-CoV-2 outbreak. Ensuring a sustained supply of clean and reusable PAPR masks for frontline medical team is an immediate challenge. The Central Sterile Supplies Unit (CSSU) adopts existing disinfection methods and technology for the reprocessing of reusable personal protective equipment (PPE) such as PAPR masks and goggles.
Objective:
To determine an effective disinfecting method for protective devices used in the course of treating SARS-CoV2-positive patients.
Method:
A comparison on surface disinfection and modified thermal disinfection outcome was conducted on 30 PAPR masks through detecting the presence of adenosine triphosphate (ATP) by swab following both disinfecting methods.
Results:
The modified thermal cycles emerged as the recommended disinfection method.
Discussion:
The outcome of this study has enhanced understanding on the risk imposed on frontline healthcare personnel who perform surface disinfecting on masks for reuse during the work shift. Leveraging on the current expertise from existing instrument logistics, CSSU takes charge of the processing and stock management of SKH's PAPR masks. An additional workflow is needed to establish reprocessing methods for other reusable PPEs such as face shields or overalls.
Related Concept Videos
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Hand hygiene
Hand washing...

