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Updated: Oct 4, 2025

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
A redox-mediator pathway for enhanced multi-colour electrochemiluminescence in aqueous solution
Emily Kerr1, David J Hayne1, Lachlan C Soulsby2
1Institute for Frontier Materials, Deakin University Geelong Victoria 3220 Australia emily.kerr@deakin.edu.au.
Abstract:
The classic and most widely used co-reactant electrochemiluminescence (ECL) reaction of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3]2+) and tri-n-propylamine is enhanced by an order of magnitude by fac-[Ir(sppy)3]3- (where sppy = 5'-sulfo-2-phenylpyridinato-C 2,N), through a novel 'redox mediator' pathway. Moreover, the concomitant green emission of [Ir(sppy)3]3-* enables internal standardisation of the co-reactant ECL of [Ru(bpy)3]2+. This can be applied using a digital camera as the photodetector by exploiting the ratio of R and B values of the RGB colour data, providing superior sensitivity and precision for the development of low-cost, portable ECL-based analytical devices.
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