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Updated: Oct 4, 2025
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
Long Non-Coding RNA and mRNA Expression Analysis in Liver of Mice With Clonorchis sinensis Infection
Su Han1, Xue-Li Zhang1, Xu Jiang1
1Department of Parasitology, Harbin Medical University, Harbin, China.
Abstract:
Clonorchiasis is recognized as an important zoonotic parasitic disease worldwide. However, the roles of host long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in the response to Clonorchis sinensis (C. sinensis) infection remain unknown. Here we compared the expression of lncRNAs and mRNAs in the liver tissue of mice infected with C. sinensis, in order to further understand the molecular mechanisms of clonorchiasis. A total of 388 lncRNAs and 1,172 mRNAs were found to be differentially expressed with absolute value of fold change (FC) ≥ 2.0 and p < 0.05 by microarray. Compared with controls, Gm6135 and 4930581F22Rik were the most over- and under-expressed lncRNAs; flavin-containing monooxygenase 3 (Fmo3) and deleted in malignant brain tumors 1 (Dmbt1) were the most over- and under-expressed mRNAs. Moreover, functional annotation showed that the significantly different mRNAs were related with "FOXO signaling pathway", "Wnt signaling pathway", and "AMPK signaling pathway". Remarkably, lncRNA Gm8801 were significantly correlated with mRNA glycerol-3-phosphate acyltransferase mitochondrial (Gpam), insulin receptor substrate 2 (Irs2), and tumor necrosis factor receptor superfamily member 19 (Tnfrsf19) in ceRNA networks. These results showed that the expression profiles of lncRNAs and mRNAs in the liver changed after C. sinensis infection. Our results provided valuable insights into the lncRNAs and mRNAs involved in clonorchiasis pathogenesis, which may be useful for future control strategies.
Insights
This study reveals significant changes in host long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in mice infected with Clonorchis sinensis. These findings offer insights into clonorchiasis pathogenesis and potential control strategies.
Area of Science:
- Parasitology
- Molecular Biology
- Genomics
Background:
- Clonorchiasis, a zoonotic disease caused by *C. sinensis*, poses a global health challenge.
- The molecular mechanisms underlying host response to *C. sinensis* infection, particularly involving host long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs), remain largely unexplored.
Purpose of the Study:
- To investigate the differential expression profiles of lncRNAs and mRNAs in mouse liver tissue following *C. sinensis* infection.
- To elucidate the molecular mechanisms and pathways involved in the host's response to clonorchiasis.
Main Methods:
- Microarray analysis was employed to compare lncRNA and mRNA expression in infected versus control mouse liver tissues.
- Differential expression analysis identified significant changes (FC ≥ 2.0, p < 0.05).
- Functional annotation and ceRNA network analysis were performed.
Main Results:
- A total of 388 lncRNAs and 1,172 mRNAs were found to be differentially expressed.
- Key differentially expressed genes included *Gm6135*, *4930581F22Rik*, *Fmo3*, and *Dmbt1*.
- Enriched pathways included FOXO, Wnt, and AMPK signaling; lncRNA *Gm8801* showed correlations with *Gpam*, *Irs2*, and *Tnfrsf19* in ceRNA networks.
Conclusions:
- *C. sinensis* infection significantly alters host lncRNA and mRNA expression profiles in the liver.
- The identified lncRNAs and mRNAs provide valuable insights into clonorchiasis pathogenesis.
- These findings may contribute to the development of novel strategies for controlling clonorchiasis.
