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Adapted Murine Sepsis Score: Improving the Research in Experimental Sepsis Mouse Model
Maicon Machado Sulzbacher1,2,3, Lucas Machado Sulzbacher1,2, Felipe Rafael Passos1
1Research Group in Physiology, Regional University of Northwestern Rio Grande do Sul State (UNIJUÍ), Ijuí, RS, Brazil.
Abstract:
The Murine Sepsis Score (MSS) is used to assess the severity of sepsis in rats and mice based on observational characteristics. The quantitative variables of glycemia, body weight, and temperature are predictors of severity in experimental models of sepsis. Therefore, our study sought to adapt the MSS with the same variables to indicate earlier the severity of the disease in murine models of the disease. Sepsis mice presented hypoglycemia, weight loss, and hypothermia. Therefore, these variables were included in the Adapted Murine Sepsis Score (A-MSS). The A-MASS presented 100% specificity and 87.5% sensibility been able to differentiate the early sepsis symptoms and its severity. The A-MSS allows an early and more complete diagnosis of sepsis in mice and might be considered as a procedure to improve the analysis of systemic sepsis dysfunction in murine experimental models.
Insights
Researchers developed an Adapted Murine Sepsis Score (A-MSS) to better assess sepsis severity in mice. This new score uses glycemia, body weight, and temperature for earlier and more accurate diagnosis of sepsis in murine models.
Area of Science:
- * Veterinary Medicine
- * Infectious Diseases
- * Animal Models
Background:
- * The Murine Sepsis Score (MSS) is a standard observational tool for assessing sepsis severity in rodents.
- * Quantitative variables like glycemia, body weight, and temperature are known predictors of sepsis severity in experimental models.
- * There is a need for earlier and more comprehensive assessment of sepsis in murine models.
Purpose of the Study:
- * To adapt the existing Murine Sepsis Score (MSS) by incorporating quantitative variables.
- * To develop an Adapted Murine Sepsis Score (A-MSS) for earlier and more accurate sepsis severity assessment in mice.
- * To evaluate the diagnostic performance of the A-MSS in murine models of sepsis.
Main Methods:
- * Inclusion of quantitative variables: glycemia, body weight, and temperature.
- * Development of the Adapted Murine Sepsis Score (A-MSS) based on these variables.
- * Evaluation of the A-MSS's specificity and sensibility in differentiating early sepsis symptoms and severity.
Main Results:
- * Sepsis in mice was characterized by hypoglycemia, weight loss, and hypothermia.
- * The Adapted Murine Sepsis Score (A-MSS) demonstrated 100% specificity and 87.5% sensibility.
- * The A-MSS effectively differentiated early sepsis symptoms and severity in murine models.
Conclusions:
- * The Adapted Murine Sepsis Score (A-MSS) provides an earlier and more complete diagnosis of sepsis in mice.
- * The A-MSS can improve the analysis of systemic sepsis dysfunction in murine experimental models.
- * This adapted score offers a valuable tool for research involving sepsis in mice.
