Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting mitochondrial protease in diffuse gliomas.

Molecular cancer therapeutics·2026
Same author

A multi-species toolkit of TOP2 hypercleavage mutants for studying topoisomerase II-mediated DNA damage.

bioRxiv : the preprint server for biology·2026
Same author

Single-cell full-length transcriptome of human lung reveals genetic effects on isoform regulation beyond gene-level expression.

bioRxiv : the preprint server for biology·2026
Same author

Evaluation of Patients Perspectives on Dental Implants and Bone Graft Surgery.

Journal of pharmacy & bioallied sciences·2026
Same author

Comparative analyses for plant virus-based cancer immunotherapy drug development.

Cell biomaterials·2026
Same author

Expanding Chemical Space of Nucleic Acid Nanoparticles for Tunable Antiviral-Like Immunomodulatory Responses and Potent Adjuvant Activity.

Advanced functional materials·2026

Related Experiment Video

Updated: Oct 4, 2025

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

4.4K

ATAC Sequencing Protocol For Cryopreserved Mammalian Cells.

Juan Manuel Caravaca1, Monika Mehta1, Sujatha Gowda1

  • 1Sequencing Facility, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.

Bio-Protocol
|February 7, 2022
PubMed
Summary

This study details an optimized Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) protocol. It refines cell thawing and magnetic bead purification for faster, efficient chromatin accessibility analysis.

Keywords:
ATAC-seqChromatin accessibilityCryopreserved cellsGenome wideNucleosome positioningTransposase

More Related Videos

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

6.3K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

12.7K

Related Experiment Videos

Last Updated: Oct 4, 2025

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues
07:12

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Published on: July 28, 2023

4.4K
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
09:09

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

6.3K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

12.7K

Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) is crucial for genome-wide chromatin accessibility and nucleosome positioning.
  • The method utilizes a hyperactive Tn5 transposase for simultaneous chromatin cutting and adapter insertion.
  • Previous iterations include OMNI ATAC-seq (Corces et al., 2017), building upon the original method (Buenrostro et al., 2013).

Purpose of the Study:

  • To present a refined ATAC-seq protocol based on the OMNI-ATAC method.
  • To optimize critical steps including thawing cryopreserved cells and magnetic bead-based library purification.
  • To provide a protocol suitable for high-throughput laboratory environments.

Main Methods:

  • Adaptation of the OMNI ATAC-seq protocol.
  • Detailed procedures for thawing cryopreserved cells.
  • Magnetic bead-based purification of ATAC-seq libraries.

Main Results:

  • The described protocol streamlines the ATAC-seq workflow.
  • Focus on optimizing initial cell handling and final library purification steps.
  • The protocol is designed for efficiency in fast-paced research settings.

Conclusions:

  • The optimized ATAC-seq protocol facilitates efficient chromatin accessibility studies.
  • The protocol addresses key steps for improved laboratory throughput.
  • This method supports rapid analysis of regulatory elements and nucleosome positioning.