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Updated: Oct 4, 2025

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid
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Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

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Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid.

Larissa Mourao1, Marta Ciwinska1, Jacco van Rheenen2

  • 1Center for Cancer Biology, VIB (BE); Department of Oncology, KU Leuven (BE).

Journal of Visualized Experiments : Jove
|February 7, 2022
PubMed
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Researchers developed a new imaging window for studying mammary gland remodeling. This R.MIW allows high-resolution, long-term optical access for detailed cellular analysis during development and disease.

Area of Science:

  • Developmental biology
  • Cellular imaging
  • Biomedical engineering

Background:

  • The mammary gland exhibits dynamic growth and remodeling post-birth.
  • Current intravital microscopy methods are limited to short observation periods (hours to days).
  • Mammary gland remodeling occurs over longer timescales (days to weeks), necessitating improved imaging techniques.

Purpose of the Study:

  • To develop an improved imaging window for long-term, high-resolution intravital microscopy of the mammary gland.
  • To enable detailed study of mammary gland cellular dynamics over extended periods.

Main Methods:

  • Development of an improved titanium mammary imaging window with a replaceable lid (R.MIW).
  • Utilized intravital microscopy for high-resolution, cellular-level imaging.

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Last Updated: Oct 4, 2025

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  • Demonstrated extended optical access for up to several weeks.

    • Main Results:

    • The R.MIW allows for high-resolution imaging of the mammary gland with cellular detail.
    • The R.MIW provides continuous tissue access for up to several weeks.
    • The R.MIW facilitates tissue manipulation, labeling, drug administration, and image-guided microdissection.

    Conclusions:

    • The R.MIW is a valuable tool for studying mammary gland remodeling and dynamics.
    • Enables high-resolution characterization of cellular dynamics during mammary gland development, homeostasis, and disease.
    • Overcomes limitations of previous imaging technologies for long-term studies.