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Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

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Published on: June 4, 2020

Organosulfur/Selenium-Based Highly Fluorogenic Molecular Probes for Live-Cell Nucleolus Imaging.

Iswar Chandra Mondal¹, Maksym Galkin^2,3, Shubham Sharma¹

¹School of Basic Sciences, Indian Institute of Technology, Mandi, H.P.-175005, India.

Chemistry, an Asian Journal

|February 7, 2022

Summary

New fluorescent probes selectively image cell nucleoli. These organoselenium and organosulfur dyes offer high contrast and enhanced emission upon binding to RNA, aiding bioimaging applications.

Keywords:

Conformational Dynamics Fluorescent Probe Nucleic Acid Nucleolus Imaging Organochalcogens

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Area of Science:

Chemical Biology
Molecular Imaging
Organic Chemistry

Background:

Developing selective fluorescent probes is crucial for advanced bioimaging.
Organochalcogen compounds offer unique photophysical properties for sensing applications.
Targeting specific cellular components like nucleoli requires tailored probe design.

Purpose of the Study:

To develop novel fluorogenic cationic organochalcogens for selective RNA imaging.
To investigate the mechanism of enhanced fluorescence upon RNA interaction.
To demonstrate the utility of these probes for high-contrast nucleoli bioimaging.

Main Methods:

Synthesis of novel organoselenium and organosulfur based fluorescent probes.
Evaluation of probe selectivity and photophysical properties in aqueous solutions.
Confocal microscopy for high-contrast imaging of cell nucleoli.
Multiscale simulation studies to understand structure-interaction relationships.

Main Results:

Developed cell-permeable, red-emissive probes with high quantum yield upon RNA binding.
Demonstrated selective and high-contrast imaging of cell nucleoli.
Simulation studies revealed RNA-induced rotational freezing and planarization enhance fluorescence via intramolecular charge transfer.
Identified the impact of heavy-chalcogens on improved emissive properties.

Conclusions:

Fluorogenic cationic organochalcogens are effective tools for selective RNA and nucleoli imaging.
Probe design incorporating cyclic sidearms and heavy-chalcogens enhances fluorescence and selectivity.
These probes offer a promising platform for advanced cellular bioimaging.