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Nucleic Acid Probe-Based Difunctional Hematology Analysis Kit for Peripheral Blood Cell Analysis
Tianping Xia1, Gengwen Chen2, Danhong Zhou1
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
ACS Sensors
|February 7, 2022
Summary
A novel nucleic acid probe, NATO, enhances hematology analysis by improving resolution and detection efficiency. This new probe offers better separation of blood cells in automated analyzers.
Area of Science:
- Hematology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Traditional "one for one channel" probes in hematology analyzers have limitations in resolution and detection efficiency.
- Short-wavelength probes are needed to overcome these limitations.
Purpose of the Study:
- To develop a novel "one for two channels" nucleic acid probe named NATO.
- To evaluate the performance of the NATO probe in an automated hematology analyzer.
Main Methods:
- Development of the NATO probe with a short wavelength (λabs = 460 nm).
- Incorporation of the NATO probe into a hematology analysis kit.
- Application of the kit in an automated hematology analyzer for cell analysis.
Main Results:
- The NATO probe demonstrated a high signal-to-noise ratio for nucleic acids.
- Short-wavelength absorbance of NATO resulted in higher resolution, improving red blood cell and platelet separation in the differentiating (DIFF) channel.
- The NATO-based kit exhibited excellent performance in both DIFF and reticulocyte channels.
Conclusions:
- The NATO probe offers improved resolution and detection efficiency compared to traditional probes.
- This novel nucleic acid probe has potential for advancing hematology analysis in automated systems.
- The NATO probe's difunctionality and high resolution contribute to enhanced blood cell differentiation.
