Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The circadian isoform landscape of mouse livers.

NAR genomics and bioinformatics·2026
Same author

Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and Chopper.

PloS one·2026
Same author

A temporal and spatial atlas of adaptive immune responses in the lymph node following viral infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multimodal single-cell analyses reveal distinct fusion-regulated transcriptional programs in Ewing sarcoma.

bioRxiv : the preprint server for biology·2025
Same author

B cell immune repertoire sequencing in tobacco cigarette smoking, vaping, and chronic obstructive pulmonary disease in the COPDGene cohort.

medRxiv : the preprint server for health sciences·2024
Same author

R2C2 + UMI: Combining concatemeric and unique molecular identifier-based consensus sequencing enables ultra-accurate sequencing of amplicons on Oxford Nanopore Technologies sequencers.

PNAS nexus·2024

Related Experiment Video

Updated: Oct 4, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K

Single-cell isoform analysis in human immune cells.

Roger Volden1, Christopher Vollmers2

  • 1Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95064, USA.

Genome Biology
|February 8, 2022
PubMed
Summary
This summary is machine-generated.

Researchers used R2C2 to analyze full-length cDNA from single cells, identifying cell types and immune receptors. This method enables detailed transcriptomics and adaptive immune receptor sequencing without relying on other data.

More Related Videos

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

11.1K
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.7K

Related Experiment Videos

Last Updated: Oct 4, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.8K
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

11.1K
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.7K

Area of Science:

  • Single-cell genomics
  • Molecular biology
  • Immunology

Background:

  • High-throughput single-cell analysis relies on protocols like 10X Genomics and Drop-Seq.
  • These methods encode transcript origin at the 5' or 3' end of cDNA.
  • Full-length transcript sequencing is crucial for comprehensive cellular analysis.

Purpose of the Study:

  • To sequence and demultiplex full-length cDNA molecules generated by the 10X Genomics platform.
  • To identify cell clusters (B cells, T cells, monocytes) and generate isoform-level transcriptomes.
  • To extract paired adaptive immune receptor sequences from individual T and B cells.

Main Methods:

  • Utilized the R2C2 sequencing technology.
  • Analyzed 12 million full-length cDNA molecules from ~3000 peripheral blood mononuclear cells.
  • Demultiplexed reads independently from Illumina data.

Main Results:

  • Successfully identified B cell, T cell, and monocyte clusters.
  • Generated high-resolution, isoform-level transcriptomes for individual cells and cell types.
  • Extracted paired adaptive immune receptor sequences, linking them to specific cells.

Conclusions:

  • R2C2 enables robust full-length cDNA sequencing and demultiplexing for single-cell analysis.
  • The method facilitates detailed transcriptomic profiling and immune receptor repertoire reconstruction.
  • This approach enhances understanding of cellular heterogeneity and immune responses.