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Related Experiment Video

Updated: Oct 4, 2025

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

An encodable multiplex microsphere-phase amplification sensing platform detects SARS-CoV-2 mutations.

Zecheng Zhong¹, Jin Wang², Shuizhen He³

¹State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen, 361102, China.

Biosensors & Bioelectronics

|February 8, 2022

Summary

This summary is machine-generated.

A new sensing platform detects SARS-CoV-2 RNA and simultaneously identifies key single-nucleotide variants (SNVs) in the receptor-binding domain. This highly sensitive method offers a clinical tool for monitoring evolving variants of concern during the COVID-19 pandemic.

Keywords:

Encodable Multiplex amplification Mutation SARS-CoV-2 Variant

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

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Published on: March 13, 2018

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

SARS-CoV-2 variants of concern (VOCs) possess single-nucleotide variants (SNVs) in the receptor-binding domain (RBD) that increase infectivity, transmission, and immune escape, worsening the COVID-19 pandemic.
The emergence of new VOCs necessitates diagnostic tools for simultaneous monitoring of these critical SNVs.
Current clinical tools lack the required sensitivity and efficiency for simultaneous SNV monitoring.

Purpose of the Study:

To develop a sensitive and efficient diagnostic platform for simultaneous detection of SARS-CoV-2 RNA and identification of key SNVs in the RBD.
To address the limitations of existing methods in monitoring rapidly evolving viral variants.

Main Methods:

Development of an encodable multiplex microsphere-phase amplification (MMPA) sensing platform.
Integration of primer-coded microsphere technology with a dual fluorescence decoding strategy.
Utilizing the amplification refractory mutation system PCR (ARMS-PCR) confined to microsphere surfaces to prevent non-specific amplification.

Main Results:

The MMPA platform achieved a low SARS-CoV-2 RNA detection limit of 28 copies/reaction.
Demonstrated the ability to detect a respiratory pathogen panel without cross-reactivity.
Achieved SNV analysis accuracy comparable to sequencing, with enhanced clinical availability.
Successfully identified 10 key SNVs in the RBD simultaneously.

Conclusions:

The MMPA platform provides a highly sensitive and efficient method for simultaneous detection of SARS-CoV-2 RNA and monitoring of critical SNVs in the RBD.
This technology offers a timely and adaptable solution for tracking evolving VOCs, surpassing the limitations of traditional sequencing methods in clinical settings.