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Effect of ozone disinfection on forensic STR profiling
Vivien Fejes1, Dominika Szucs1, Katalin Sipos2
1Department of Forensic Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
Forensic Science International
|February 8, 2022
Summary
Ozone treatment effectively disinfects forensic evidence without inhibiting DNA amplification. This method shows promise for forensic genetics, as it maintains full short tandem repeat (STR) profiles on treated samples.
Area of Science:
- Forensic Science
- Microbiology
- Genetics
Background:
- Forensic laboratories require effective disinfection methods to prevent pathogen transmission.
- Current disinfection techniques are limited in their suitability for forensic genetic analysis.
- Ideal methods must preserve DNA amplification and work on porous surfaces.
Purpose of the Study:
- To evaluate ozone treatment as a disinfection method for forensic samples.
- To determine if ozone treatment affects DNA amplification and short tandem repeat (STR) profiling.
- To assess the efficacy of ozone on extracted DNA and mock casework samples.
Main Methods:
- Ozone treatment applied to extracted DNA and mock casework samples (surgical masks).
- DNA amplification using Qiagen Investigator ESSplex SE QS kit.
- Comparison of detected allele counts between ozone-treated and control samples.
Main Results:
- Ozone treatment reduced DNA concentration by half but yielded full STR profiles.
- No significant difference in allele counts between treated and untreated mock casework samples (p=0.513).
- Sampling location and contributor variations had a greater impact on allele counts than ozone treatment.
Conclusions:
- Ozone treatment is a viable disinfection method for forensic DNA analysis.
- The technique does not hinder routine forensic DNA analysis.
- Further research may explore optimal application parameters for diverse forensic evidence types.
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