Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flavonoids from <i>Acer okamotoanum</i> Inhibit Adipocyte Differentiation and Promote Lipolysis in the 3T3-L1 Cells.

Molecules (Basel, Switzerland)·2020
Same author

Clinical Practice Guideline for Endoscopic Resection of Early Gastrointestinal Cancer.

Clinical endoscopy·2020
Same author

Polymorphisms in Glycolysis-Related Genes Are Associated with Clinical Outcomes of Paclitaxel-Cisplatin Chemotherapy in Non-Small Cell Lung Cancer.

Oncology·2020
Same author

Improved detection of hepatocellular carcinoma by dynamic computed tomography in cirrhotic patients with chronic hepatitis B: A multicenter study.

Journal of gastroenterology and hepatology·2020
Same author

Development and growth of auricular cartilage and muscles: A study using human fetuses.

International journal of pediatric otorhinolaryngology·2020
Same author

The incudopetrosal joint of the human middle ear: a transient morphology in fetuses.

Journal of anatomy·2020

Related Experiment Video

Updated: Oct 4, 2025

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

26.7K

Complement C5aR/LPS-induced BDNF and NGF modulation in human dental pulp stem cells.

Muhammad Irfan1, Ji Hyun Kim1, Robert E Druzinsky1

  • 1Department of Oral Biology, College of Dentistry, University of Illinois Chicago, 801 S. Paulina St, Chicago, IL, 60612, USA.

Scientific Reports
|February 8, 2022
PubMed
Summary

Dental pulp stem cells (DPSCs) show enhanced nerve regeneration potential. The complement C5a receptor (C5aR) pathway, modulated by inflammation, significantly boosts brain-derived neurotrophic factor (BDNF) production in DPSCs.

More Related Videos

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

7.5K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

1.9K

Related Experiment Videos

Last Updated: Oct 4, 2025

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

26.7K
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

7.5K
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
02:33

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Published on: May 17, 2024

1.9K

Area of Science:

  • Regenerative Medicine
  • Neuroscience
  • Immunology

Background:

  • Stem cells offer promise for nerve regeneration due to their differentiation and secretion capabilities.
  • Dental pulp stem cells (DPSCs) are accessible and possess neural repair qualities, making them suitable for nerve regeneration.
  • The complement C5a system is a key mediator in inflammation and tissue regeneration.

Purpose of the Study:

  • To investigate the role of the complement C5a receptor (C5aR) in modulating brain-derived neurotrophic factor (BDNF) production in DPSCs.
  • To compare the neurogenic potential of DPSCs with bone marrow-derived mesenchymal stem cells (BM-MSCs) under inflammatory conditions.
  • To elucidate the interplay between inflammation, C5aR, and BDNF/nerve growth factor (NGF) in DPSCs.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) and immunostaining were used to analyze BDNF production.
  • C5aR was blocked or activated using specific agonists and antagonists.
  • DPSCs and BM-MSCs were stimulated with lipopolysaccharide (LPS) to induce inflammation.

Main Results:

  • Blocking C5aR reduced BDNF production in DPSCs, while C5aR agonist treatment increased it, indicating a positive regulatory role.
  • Inflammation induced by LPS potentiated C5aR-mediated BDNF production in a C5aR-dependent manner.
  • DPSCs exhibited significantly higher C5aR-mediated BDNF levels compared to BM-MSCs.

Conclusions:

  • C5aR plays a crucial role in regulating BDNF production in DPSCs.
  • Inflammation enhances C5aR-mediated BDNF secretion in DPSCs.
  • DPSCs demonstrate superior neurogenic potential compared to BM-MSCs, particularly under inflammatory stimulation, highlighting their therapeutic value in nerve regeneration.