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[Diagnostic and predictive markers in urinary tract cytology]
Tatjana Vlajnic1, Lukas Bubendorf2
1Pathologie, Institut für medizinische Genetik und Pathologie, Universitätsspital Basel, Schönbeinstr. 40, 4031, Basel, Schweiz. Tatjana.Vlajnic@usb.ch.
Der Pathologe
|February 8, 2022
Summary
Multiprobe fluorescence in situ hybridization (FISH) is the gold standard for urinary cytology atypia. The Paris System guides FISH use, integrating morphology and molecular diagnostics for future advancements like next-generation sequencing.
Area of Science:
- Uropathology
- Cytogenetics
- Molecular Diagnostics
Background:
- Multiprobe fluorescence in situ hybridization (FISH) is crucial for diagnosing inconclusive urinary cytology.
- The Paris System (TPS) provides a framework for utilizing FISH and integrating morphological findings.
Purpose of the Study:
- To highlight the role of FISH in urinary cytology.
- To discuss the integration of FISH with morphological analysis.
- To explore the future potential of next-generation sequencing (NGS) in urinary diagnostics.
Main Methods:
- Review of current diagnostic standards in urinary cytology.
- Application of The Paris System guidelines for FISH testing.
- Discussion of emerging molecular technologies.
Main Results:
- FISH remains the established method for resolving ambiguous urinary cytology cases.
- Morphological correlation is essential for an accurate diagnostic approach.
- Next-generation sequencing shows promise for detecting multiple genetic alterations in urine specimens.
Conclusions:
- FISH is indispensable in current urinary cytology practice for inconclusive atypia.
- An integrative diagnostic approach combining morphology and FISH is emphasized.
- Next-generation sequencing is poised to become a significant diagnostic tool in the near future.
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