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Updated: Oct 4, 2025

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
Ulcerating nodules on a background of lower leg lymphoedema
Mia Steyn1, Nastassia Nardini1, Natalie Rachel Attard1
1St John's Institute of Dermatology, Guy's and St Thomas' NHS Foundation Trust, London, UK.
Abstract:
A patient presented with a 3-month history of a rapidly enlarging ulcerated tumour on his lower leg, occurring on a background of chronic idiopathic lymphoedema of approximately 10 years' duration. Histology revealed extensive infiltration of the dermis by a vascular tumour with pleomorphic and hyperchromatic endothelial cells, which stained positive for vascular markers CD31, CD34 and ERG. A diagnosis of lymphoedema-associated angiosarcoma was reached and our patient was treated with isolated limb perfusion with high-dose melphalan and tumour necrosis factor-alfa.
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