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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Oct 4, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

Temporal analyses of postnatal liver development and maturation by single-cell transcriptomics.

Yan Liang¹, Kota Kaneko¹, Bing Xin¹

¹Department of Pathology, Division of Biological Sciences, and Moores Cancer Center, University of California, San Diego, La Jolla, CA 92093, USA.

Developmental Cell

|February 8, 2022

Summary

This study maps mouse liver development using single-cell transcriptomes, revealing dynamic cell changes and metabolic zonation. It identifies key transcription factors and unique macrophage populations crucial for liver maturation and function.

Keywords:

construction of metabolic zones endothelial cells functional maturation of liver hepatocyte heterogeneity interaction of macrophages postnatal liver development single cell transcriptomics

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Last Updated: Oct 4, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

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Area of Science:

Developmental Biology
Hepatology
Genomics

Background:

Liver development and maturation are complex processes that remain incompletely understood.
The liver's role as a major metabolic organ necessitates a detailed understanding of its cellular development.

Purpose of the Study:

To comprehensively analyze the cellular landscape of the developing mouse liver.
To identify key cell types, states, and molecular regulators involved in postnatal liver maturation.

Main Methods:

Analysis of 52,834 single-cell transcriptomes from mouse livers at multiple postnatal time points (days 1, 3, 7, 21, 56).
Utilized trajectory and gene regulatory network analyses to identify developmental programming factors.

Main Results:

Identified 31 distinct cell types or states within the developing liver.
Observed significant hepatocyte heterogeneity and progressive zonation of metabolic functions.
Discovered a novel macrophage population with hybrid phenotypes potentially involved in sinusoidal development and immune regulation.
Identified 36 transcription factors, including Bhlhe40, critical for liver development.

Conclusions:

This study provides a comprehensive single-cell atlas of liver development, offering insights into cellular heterogeneity and metabolic zonation.
The findings highlight novel cell populations and regulatory mechanisms essential for understanding liver maturation and disease.
The atlas serves as a valuable resource for future research in liver biology and regenerative medicine.