Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of Fitbit-Based Interventions in Improving 24-hour Movement Behaviors: A Systematic Review and Meta-Analysis.

Inquiry : a journal of medical care organization, provision and financing·2026
Same author

Photoinduced, Energy-Transfer-Mediated Synergistic N,S-Difunctionalization of Alkenes with CN Migration.

Organic letters·2026
Same author

Expression and ligand-binding characterization of two chemosensory proteins (TabsCSP8 and TabsCSP19) in Tuta absoluta.

Pesticide biochemistry and physiology·2026
Same author

Histone chaperone HIRA restrains ferroptosis susceptibility through epigenetic control of iron metabolism in prostate cancer.

Science China. Life sciences·2026
Same author

The legs of the young heart stool: how motor competence, muscle strength, and physical activity support cardiorespiratory fitness in preschoolers?

Frontiers in sports and active living·2026
Same author

Cytoreductive radical prostatectomy in patients with high-volume metastatic prostate cancer achieving deep biochemical response to contemporary systemic therapy: a multicentre, prospective cohort study.

BMC medicine·2026

Related Experiment Video

Updated: Oct 4, 2025

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.5K

Full-length codling moth transcriptome atlas revealed by single-molecule real-time sequencing.

Longsheng Xing1, Qiang Wu1, Yu Xi1

  • 1Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.

Genomics
|February 8, 2022
PubMed
Summary
This summary is machine-generated.

Pacific Biosciences sequencing revealed novel full-length transcripts and alternative splicing in codling moth, advancing transcriptome complexity understanding and genome annotation.

Keywords:
Alternative splicingCydia pomonellaFull-length transcriptomeFusion geneIsoSeq

More Related Videos

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.6K
RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

7.9K

Related Experiment Videos

Last Updated: Oct 4, 2025

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.5K
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

17.6K
RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

7.9K

Area of Science:

  • Transcriptomics
  • Insect Genomics
  • Molecular Biology

Background:

  • Second-generation sequencing (SGS) has limitations in defining full-length (FL) transcripts and alternative splice (AS) isoforms.
  • Accurate transcriptome elucidation is crucial for understanding gene function and regulation in insects.

Purpose of the Study:

  • To obtain high-quality FL transcriptome data in the codling moth using PacBio sequencing.
  • To identify novel transcripts, genes, and alternative splicing events.
  • To create a comprehensive FL transcript atlas for the codling moth.

Main Methods:

  • Pacific Biosciences (PacBio) single-molecule real-time sequencing for FL transcriptome data acquisition.
  • Clustering of obtained isoforms to identify nonredundant transcripts.
  • Validation of identified transcripts, including lncRNAs and fusion transcripts, using RT-PCR.

Main Results:

  • Generated 25,940 high-quality FL isoforms, clustered into 14,099 nonredundant clusters.
  • Approximately 90% of nonredundant transcripts were novel compared to reference genes, with 3389 potentially novel genes identified.
  • Discovered numerous AS events and identified 952 FL lncRNAs and 81 fusion transcripts.

Conclusions:

  • PacBio sequencing provides a more comprehensive view of the codling moth transcriptome than SGS.
  • The generated FL transcript atlas offers valuable insights into transcriptome complexity and aids in improving genome annotations.
  • This study facilitates future functional studies of genes and transcripts in the codling moth.