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Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

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Insights into gene manipulation techniques for Acari functional genomics.

Beatrice T Nganso¹, Gur Pines¹, Victoria Soroker¹

¹Department of Entomology, Chemistry and Nematology, Institute of Plant Protection, Agricultural Research Organization, The Volcani Centre, Rishon LeZion, Israel.

Insect Biochemistry and Molecular Biology

|February 8, 2022

Summary

Functional genomics in Acari (mites and ticks) utilizes gene manipulation tools like RNA interference (RNAi) and CRISPR-Cas9. This review compares their effectiveness and challenges for understanding mite and tick gene functions.

Keywords:

CRISPR Chelicerata Gene editing Gene silencing RNAi

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Published on: July 1, 2021

Area of Science:

Genomics
Molecular Biology
Entomology

Background:

Acari (mites and ticks) impact agriculture, health, and ecosystems.
Functional genomics is crucial for understanding these organisms.
Gene manipulation techniques are key tools in functional genomics.

Purpose of the Study:

To review gene manipulation techniques in Acari functional genomics.
To compare RNA interference (RNAi) and CRISPR-Cas9 systems.
To discuss challenges and potential solutions for gene function studies in Acari.

Main Methods:

Literature review of gene manipulation techniques in Acari.
Comparison of RNA interference (RNAi) and CRISPR-Cas9 technologies.
Analysis of technical challenges and optimization strategies.

Main Results:

RNA interference (RNAi) is currently the primary tool, but with variable outcomes.
CRISPR-Cas9 systems are emerging but limited to CRISPR/Cas9 in Acari.
Both techniques face specific challenges in Acari species.

Conclusions:

Optimizing RNAi and CRISPR-Cas9 is essential for advancing Acari functional genomics.
Exploring diverse CRISPR-Cas9 systems can expand research capabilities.
Understanding gene function in Acari is vital for managing pests and appreciating beneficial species.