Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Signalling through RXRβ and its agonist, bexarotene, promotes neuron formation in Xenopus laevis embryos.

Biology open·2026
Same author

Modelling human genetic disorders in Xenopus tropicalis.

Disease models & mechanisms·2024
Same author

CRISPR/Cas9 Gene Disruption Studies in F<sub>0</sub> Xenopus Tadpoles: Understanding Development and Disease in the Frog.

Methods in molecular biology (Clifton, N.J.)·2023
Same author

Genetics and Gene Editing Methods in <i>Xenopus laevis</i> and <i>Xenopus tropicalis</i>.

Cold Spring Harbor protocols·2022
Same author

An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus.

Developmental biology·2021
Same author

Cryopreservation of <i>Xenopus</i> Sperm and In Vitro Fertilization Using Frozen Sperm Samples.

Cold Spring Harbor protocols·2021

Related Experiment Video

Updated: Oct 4, 2025

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

12.0K

I-SceI-Mediated Transgenesis in Xenopus.

Anna Noble1, Anita Abu-Daya1, Matthew J Guille2

  • 1European Xenopus Resource, School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom.

Cold Spring Harbor Protocols
|February 9, 2022
PubMed
Summary

Efficient transgenic frog generation is possible using I-SceI meganuclease. This method, involving DNA digestion and injection into fertilized eggs, achieves high integration rates of up to 70%.

More Related Videos

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

22.8K
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
06:32

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

6.4K

Related Experiment Videos

Last Updated: Oct 4, 2025

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

12.0K
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

22.8K
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
06:32

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

Published on: September 4, 2019

6.4K

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Transgenic animal generation is crucial for biological research.
  • Efficient and reliable methods for creating transgenic organisms are in demand.
  • The use of specific nucleases can facilitate targeted genomic integration.

Purpose of the Study:

  • To evaluate the efficiency of I-SceI meganuclease for generating transgenic frogs.
  • To describe the methodology for using I-SceI meganuclease in frog transgenesis.

Main Methods:

  • Transgenes were prepared flanked by I-SceI recognition sites on plasmids or PCR products.
  • In vitro digestion of the transgene construct using I-SceI meganuclease.
  • Injection of the digested construct into fertilized frog eggs.
  • Assessment of transgene integration and post-transgenesis development.

Main Results:

  • I-SceI meganuclease enables highly efficient generation of transgenic frogs.
  • Transgene integration rates of up to 70% were achieved.
  • Post-transgenesis development in the frog embryos was typically normal.

Conclusions:

  • I-SceI meganuclease is a powerful tool for efficient frog transgenesis.
  • The described method offers a reliable approach for creating transgenic frog models.
  • Further research may elucidate the precise mechanism of I-SceI-mediated integration.