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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

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PTBP3 modulates P53 expression and promotes colorectal cancer cell proliferation by maintaining UBE4A mRNA stability

Canbin Xie1, Fei Long1, Liang Li1

  • 1Department of Gastrointestinal Surgery, The Third XiangYa Hospital of Central South University, Changsha, Hunan, 410013, China.

Cell Death & Disease
|February 9, 2022
PubMed

Insights

The RNA binding protein PTBP3 promotes colorectal cancer (CRC) growth by stabilizing UBE4A, which degrades P53. PTBP3 may serve as a prognostic biomarker and therapeutic target for CRC patients.

Area of Science:

  • Molecular Oncology
  • RNA Biology

Background:

  • The RNA binding protein PTBP3 is implicated in various cancers.
  • Its specific role in colorectal cancer (CRC) is not well understood.

Purpose of the Study:

  • To investigate the role and molecular mechanisms of PTBP3 in colorectal cancer (CRC).

Main Methods:

  • PTBP3 expression analysis in CRC tissues.
  • PTBP3 knockdown in CRC cell lines (in vitro and in vivo).
  • Analysis of UBE4A and P53 expression and interaction with PTBP3 mRNA.

Main Results:

  • PTBP3 is upregulated in CRC and correlates with poor prognosis.
  • PTBP3 knockdown inhibits CRC cell proliferation.
  • PTBP3 stabilizes UBE4A mRNA, leading to P53 degradation.
  • UBE4A overexpression rescues PTBP3 knockdown effects.

Conclusions:

  • PTBP3 promotes CRC proliferation by stabilizing UBE4A and regulating P53.
  • PTBP3 is a potential prognostic biomarker and therapeutic target for CRC.

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