Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insulin processing by cultured hepatocytes.

W G Blackard, R M Smith, L Jarett

    The American Journal of Physiology
    |February 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mechanisms of nuclear translocation of insulin.

    Cell biochemistry and biophysics·2000
    Same author

    Differential modulation of the tyrosine phosphorylation state of the insulin receptor by IRS (insulin receptor subunit) proteins.

    Molecular endocrinology (Baltimore, Md.)·1999
    Same author

    Angiotensin II inhibits insulin-induced egr-1 expression in mesangial cells.

    Archives of biochemistry and biophysics·1999
    Same author

    Insulin-induced protein tyrosine phosphorylation cascade and signalling molecules are localized in a caveolin-enriched cell membrane domain.

    Cellular signalling·1998
    Same author

    Receptor-mediated cellular entry of nuclear localizing anti-DNA antibodies via myosin 1.

    The Journal of clinical investigation·1997
    Same author

    Nuclear localization of autoantibodies. Novel insights into protein translocation and cellular function.

    Annals of the New York Academy of Sciences·1997
    Same journal

    Blood coagulation in fish.

    The American journal of physiology·2011
    Same journal

    Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

    The American journal of physiology·2010
    Same journal

    The inactivation of placental toxin by human serum.

    The American journal of physiology·2010
    Same journal

    Adrenal function following ovariectomy in the rat.

    The American journal of physiology·2010
    Same journal

    Capillary permeability; perfusion of frog and guinea pig hind limbs.

    The American journal of physiology·2010
    Same journal

    Evaluation of protective measures against sunburn.

    The American journal of physiology·2010
    See all related articles

    Chloroquine inhibits early insulin degradation in hepatocytes at 37°C, but this effect is masked by rapid processing at physiological temperatures. The chloroquine-sensitive step is not essential for overall insulin breakdown by liver cells.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hepatology

    Background:

    • Insulin degradation is a crucial process for regulating blood glucose levels.
    • Hepatocytes play a significant role in insulin metabolism.
    • The specific mechanisms and temperature-dependent steps of insulin degradation are not fully elucidated.

    Purpose of the Study:

    • To investigate the chloroquine-sensitive and chloroquine-insensitive steps in insulin degradation by cultured hepatocytes.
    • To understand the role of temperature in modulating these degradation pathways.
    • To determine if chloroquine's inhibitory effect on insulin degradation is obligatory at physiological temperatures.

    Main Methods:

    • Utilizing cultured hepatocytes to study insulin degradation.
    • Employing low temperatures (15°C) to slow down cellular processing.

    Related Experiment Videos

  • Varying association and dissociation times to examine early versus later stages of degradation.
  • Quantifying insulin degradation and analyzing the volume density of multivesicular bodies (MVBs) in the presence of chloroquine.

    • Main Results:

    • Chloroquine inhibited insulin degradation at 15°C and during early stages (first 15 min) at 37°C.
    • The inhibitory effect of chloroquine at 37°C was masked under standard, longer incubation conditions.
    • Chloroquine increased slowly dissociable insulin and the volume density of MVBs at both 15°C and 37°C.
    • Rapid processing of insulin from a slowly dissociable compartment at 37°C obscured chloroquine's effect.

    Conclusions:

    • The chloroquine-sensitive step in insulin degradation by hepatocytes is not obligatory at physiological temperatures.
    • Rapid insulin processing at 37°C masks the inhibitory effects of chloroquine observed at lower temperatures or shorter incubation times.
    • Multivesicular bodies are involved in the insulin degradation pathway influenced by chloroquine.