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Related Experiment Videos

A method for large volume blood sampling and transfusion in rats.

H R Berthoud, W B Laughton, T L Powley

    The American Journal of Physiology
    |March 1, 1986
    PubMed
    Summary

    This study introduces a rapid, large-volume blood withdrawal protocol for rats using indwelling catheters. The method ensures physiological stability, making it suitable for various research applications.

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    Area of Science:

    • Physiology
    • Animal Research Methods

    Background:

    • Accurate physiological measurements in rats often require significant blood volume withdrawal.
    • Existing methods can be time-consuming and may induce physiological stress.

    Purpose of the Study:

    • To develop and validate a novel protocol for rapid, large-volume whole blood withdrawal in rats.
    • To assess the physiological impact of this blood withdrawal method on rats.

    Main Methods:

    • Utilized indwelling catheters for blood withdrawal from the inferior vena cava and isovolemic replacement into the superior vena cava.
    • Employed a multiple-channel peristaltic pump to ensure simultaneous withdrawal and replacement with precise volume control.
    • Validated the protocol in both anesthetized and unanesthetized rats.

    Main Results:

    • Successfully withdrew large blood volumes from rats in under one hour.
    • Demonstrated that key physiological parameters (blood pressure, heart rate, hematocrit) remained stable.
    • Observed no significant changes in plasma hormones (insulin, glucagon), glucose levels, or feeding behavior.

    Conclusions:

    • The developed protocol enables efficient, large-volume blood sampling in rats with minimal physiological disturbance.
    • This method offers a reliable tool for physiological research requiring substantial blood collection.
    • Considerations for technical and physiological limitations are discussed to guide implementation.

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