Three-dimensional observation of the intrahepatic lymphatics by scanning electron microscopy of corrosion casts

The three-dimensional arrangement of intrahepatic lymphatics was demonstrated in the rabbit liver by scanning electron microscopy of corrosion casts. These casts were prepared by injecting resin into the common bile duct at a pressure that caused resin to leak from the bile ducts in the small portal tracts and drain into the lymphatics. The lymphatic channels were composed of straight vessels and anastomosing short side branches. The anastomoses were especially rich at the bifurcation of the portal tracts and formed a network. The terminal branches were blind-ended. Constrictions suggesting valves were occasionally observed.