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Injured coliforms in drinking water.

G A McFeters, J S Kippin, M W LeChevallier

    Applied and Environmental Microbiology
    |January 1, 1986
    PubMed
    Summary

    Most drinking water coliforms are injured and missed by standard tests. A new method, m-T7 agar, detected significantly more coliforms, highlighting the need for improved water quality monitoring.

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    Area of Science:

    • Environmental microbiology
    • Water quality analysis
    • Public health microbiology

    Background:

    • Coliforms are key indicators of drinking water contamination.
    • Conventional enumeration methods may underestimate coliform presence due to cell injury.
    • Understanding injured coliform occurrence is crucial for accurate risk assessment.

    Purpose of the Study:

    • To investigate the prevalence and impact of injured coliforms in community drinking water systems.
    • To compare the efficacy of m-T7 agar with m-Endo agar LES for coliform detection.
    • To assess the significance of injured coliforms in routine water quality monitoring.

    Main Methods:

    • Enumeration of coliforms using m-Endo agar LES and m-T7 agar.
    • Analysis of 102 routine drinking water samples from three New England community water systems.
    • Sampling across various points including post-treatment, distribution system, and post-repair.

    Main Results:

    • Injured coliforms constituted over 95% of detected coliforms.
    • m-T7 agar identified 8- to 38-fold more coliforms than m-Endo agar LES.
    • Standard methods (m-Endo agar LES) failed to detect coliforms present in samples positive by m-T7 agar.

    Conclusions:

    • A significant proportion of coliforms in the studied drinking water systems were injured.
    • Conventional analytical media (m-Endo agar LES) largely failed to detect these injured coliforms.
    • m-T7 agar offers a more sensitive method for detecting coliforms, including injured ones, in drinking water.

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