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Related Experiment Video

Updated: Oct 4, 2025

Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies

Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies

Published on: April 12, 2022

Sampling method comparison of enterococci aerosolization during continuous bubble bursting generation.

Peter J Sahwell¹, Afeefa Abdool-Ghany¹, James Klaus²

¹Department of Civil, Architectural, and Environmental Engineering, College of Engineering, University of Miami, Coral Gables, FL 33146, USA.

FEMS Microbiology Letters

|February 9, 2022

Summary

Aerosol filters effectively capture viable fecal bacteria transferred from water to air, outperforming impactors in recovery. Both methods showed similar variability in collecting these environmental aerosols.

Keywords:

aerosol bioaerosol enterococci filtration impaction pathogen

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Area of Science:

Environmental microbiology
Aerosol science
Water quality monitoring

Background:

Fecal indicator bacteria, such as enterococci, can transfer from water to air.
Understanding this water-to-air transfer is crucial for assessing environmental health risks, especially in areas with contaminated recreational waters.

Purpose of the Study:

To investigate the water-to-air transfer of viable enterococci.
To compare the capture efficiency of an impactor versus an aerosol filter for collecting these bacteria.

Main Methods:

A simulated air-sea interface was used to aerosolize enterococci.
Viable enterococci were collected using both an impactor and an aerosol filter.
Bacterial concentrations were quantified as colony-forming units (CFU)/L.

Main Results:

The aerosol filter captured significantly more viable enterococci (171.2 CFU/L) than the impactor (70.1 CFU/L).
Both methods yielded comparable variability, with coefficients of variation of 14% for the filter and 13% for the impactor.
The study confirmed viable enterococci transfer across the air-sea interface.

Conclusions:

Aerosol filters demonstrate superior performance for collecting viable, aerosolized enterococci compared to impactors.
Both methods are suitable for capturing viable bacteria, but filters offer higher recovery rates.
Findings are relevant for monitoring environmental aerosols from contaminated water sources.