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Updated: Oct 4, 2025

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019
Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation
Kelly M Cautivo1, Peri R Matatia1, Carlos O Lizama2
1Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.
Immune cells called group 2 innate lymphoid cells (ILC2s) and type 2 helper T (Th2) cells can move into tissues during allergic reactions. However, inflammation from other immune responses can block this movement, protecting against certain infections.
Area of Science:
- Immunology
- Cell Biology
- Tissue Architecture
Background:
- Group 2 innate lymphoid cells (ILC2s) and type 2 helper T (Th2) cells are key players in allergic immunity, typically found at barrier sites.
- These cells also reside in fibroblast-rich adventitial layers of vessels and deep tissues, but their dynamic repositioning during immune challenges is not well understood.
Purpose of the Study:
- To investigate the dynamic repositioning of ILC2s and Th2 cells in response to different inflammatory conditions.
- To determine the role of mixed type 1 and type 2 inflammation on ILC2 and Th2 cell trafficking and survival.
Main Methods:
- Utilized thick-section quantitative imaging to analyze immune cell distribution in lung and liver tissues.
- Investigated the impact of type 1 inflammation, specifically Interferon-gamma (IFNγ), on ILC2 and Th2 cell behavior.
Main Results:
- Allergic inflammation induced ILC2 and Th2 cell invasion into lung and liver non-adventitial parenchyma.
- Concurrent type 1 inflammation, mediated by IFNγ, inhibited ILC2 and Th2 cell parenchymal trafficking and survival.
- Confinement of ILC2 and Th2 cells to adventitia protected against mortality from Listeria monocytogenes infection.
Conclusions:
- Tissue lymphocyte subset topography is dynamically regulated during immune responses.
- IFNγ-producing type 1 lymphocytes can restrict ILC2 and Th2 cell tissue invasion and survival.
- Spatial positioning of immune cells is crucial for balanced immunity and host defense.
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10:10Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Published on: November 16, 2016
10:00High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
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