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Related Experiment Video

Updated: Oct 4, 2025

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium
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Shedding Light on ecDNA Behavior Using CRISPR-Based Live-Cell Imaging.

Anton G Henssen1,2,3,4

  • 1Department of Pediatric Oncology/Hematology, Charité-Universitätsmedizin Berlin, Berlin, Germany. henssenlab@gmail.com.

Cancer Discovery
|February 10, 2022
PubMed
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Scientists developed ecTag, a CRISPR-based tool to visualize extrachromosomal DNA (ecDNA) in live cancer cells. This method revealed how ecDNA segregates randomly during cell division and forms active hubs post-mitosis.

Area of Science:

  • Molecular biology
  • Genetics
  • Cancer research

Background:

  • Extrachromosomal DNA (ecDNA) harboring oncogenes is frequently amplified in various cancers.
  • Understanding ecDNA dynamics is crucial for cancer biology and therapeutic development.

Purpose of the Study:

  • To develop a novel method for real-time imaging of ecDNA in live cells.
  • To investigate the behavior and organization of ecDNA during the cell cycle in cancer cells.

Main Methods:

  • Development of ecTag, a CRISPR-based system for fluorescently labeling and imaging ecDNA.
  • Live-cell imaging techniques to observe ecDNA dynamics in cancer cells.

Main Results:

  • ecTag successfully visualized ecDNA in live cancer cells.

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  • Demonstrated random mitotic segregation of ecDNA molecules.
  • Observed clustering of ecDNA into transcriptionally active hubs after mitosis.

    • Conclusions:

    • ecTag provides a powerful tool for studying ecDNA in real-time.
    • The findings offer new insights into the cell cycle behavior and functional organization of ecDNA in cancer.