Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-linked helicases DDX3X and DDX3Y regulate G-quadruplex-associated stress in neurons.

Cell death & disease·2026
Same author

1950-2000: five decades of curiosity-driven discovery in alternative DNA structures.

Nucleic acids research·2026
Same author

DNA damaging properties of G-quadruplex ligand QN-302 are potentiated by the DNA repair inhibitor Olaparib and mitigated by the molecular helicase PhpC.

Genome biology·2026
Same author

Designing an ecofriendly catalyst for a sustainable use of water resources.

National science review·2026
Same author

Photoactivatable, Biomimetic Ligand photoMultiTASQ Traps DNA/RNA G‑Quadruplexes and Their Protein Binding Partners.

ACS bio & med chem Au·2025
Same author

Age-associated G-quadruplex accumulation and DDX5 loss shape chromatin landscapes in human astrocytes.

Research square·2025

Related Experiment Video

Updated: Oct 4, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.5K

Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq.

Sunny Y Yang1, David Monchaud2, Judy M Y Wong3

  • 1Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada.

Nature Protocols
|February 10, 2022
PubMed
Summary

This study introduces a G-quadruplex RNA precipitation (G4RP) method for transcriptomic profiling. G4RP uses chemical cross-linking and a specific probe to capture and analyze folded G-quadruplex RNAs.

More Related Videos

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.7K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.2K

Related Experiment Videos

Last Updated: Oct 4, 2025

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.5K
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.7K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.2K

Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Guanine-rich RNAs form G-quadruplexes (G4-RNAs), which are involved in various biological functions.
  • Understanding the transcriptome of G4-RNAs is crucial for deciphering their roles.

Purpose of the Study:

  • To detail a novel G-quadruplex RNA-specific precipitation (G4RP) protocol for transcriptomic profiling.
  • To enable the global snapshotting and analysis of folded G4-RNAs and their dynamic changes.

Main Methods:

  • The G4RP protocol involves chemical cross-linking of G4-RNAs.
  • Affinity capture is performed using the G4-specific probe, BioTASQ.
  • G4RP can be coupled with sequencing for comprehensive transcriptomic analysis.

Main Results:

  • The G4RP protocol provides a detailed method for transcriptomic profiling of G4-RNAs.
  • The method allows for capturing a global snapshot of folded G4-RNAs.
  • G4RP can profile changes in the G4-RNA transcriptome, such as those induced by G4 ligand treatments.

Conclusions:

  • G4RP is an effective method for studying G4-RNAs and their transcriptome.
  • The protocol is adaptable, completable within 1-2 weeks, and scalable.
  • A guide for optimizing the protocol in various lab settings is provided.