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Related Experiment Video

Updated: Oct 4, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
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Uncoupling Molecular Testing for SARS-CoV-2 From International Supply Chains.

Jo-Ann L Stanton1, Rory O'Brien1,2, Richard J Hall3

  • 1Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.

Frontiers in Public Health
|February 10, 2022
PubMed
Summary

New Zealand scientists developed the HomeBrew (HB) real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay using locally sourced reagents. This demonstrates the nation's capability to produce its own COVID-19 diagnostic components if global supply chains fail.

Keywords:
COVID-19HomeBrewRT-qPCRmolecular reagentssupply chain

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Area of Science:

  • Molecular Diagnostics
  • Epidemiology
  • Biotechnology

Background:

  • The COVID-19 pandemic highlighted global reliance on limited suppliers for critical RT-qPCR reagents.
  • Supply chain disruptions, exacerbated by lockdowns, severely impacted reagent availability, particularly for island nations like New Zealand.
  • This prompted an investigation into New Zealand's domestic capacity for producing essential diagnostic reagents.

Purpose of the Study:

  • To assess New Zealand's capability to manufacture COVID-19 RT-qPCR reagents domestically.
  • To develop a functional COVID-19 diagnostic assay using solely onshore-sourced components.
  • To evaluate the performance of this self-sufficient assay against commercial standards.

Main Methods:

  • Reviewed New Zealand's specific requirements for COVID-19 RT-qPCR testing.
  • Assembled local expertise and resources to synthesize all necessary reagents for RT-qPCR.
  • Developed and validated the HomeBrew (HB) RT-qPCR assay using clinical samples.

Main Results:

  • Successfully synthesized all required reagents for RT-qPCR from onshore components.
  • Developed the HomeBrew (HB) RT-qPCR assay, a one-step diagnostic tool.
  • Clinical evaluation demonstrated the HB RT-qPCR assay performance was comparable to a commercial COVID-19 assay.

Conclusions:

  • New Zealand possesses the scientific expertise to produce its own COVID-19 diagnostic reagents.
  • With adequate planning, the nation has the infrastructure capacity to overcome reagent supply challenges.
  • The development of the HB RT-qPCR assay provides a model for self-sufficiency in molecular diagnostics during crises.