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Updated: Oct 4, 2025
Time-lapse Live Imaging and Quantification of Fast Dendritic Branch Dynamics in Developing Drosophila Neurons
Published on: September 25, 2019
Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes
Guilherme O Barbosa1, Thomas B Kornberg1
1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA.
Abstract:
This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-Studios pipeline. It can also be used for studies of cell division, organelle localization, and protein trafficking as well as other cellular materials that can be fluorescently tagged and imaged with minimal phototoxicity.
