Source-dependent variation in phenolic compounds and antioxidant activities of Prinsepia utilis Royle fruits

Prinsepia utilis, a wild-growing Himalayan shrub, is a good source of phytoceuticals, cosmeceuticals, and antioxidants. The present study is an attempt to investigate the effect of altitude and edaphic factors on phenolics and antioxidant activity in fruit pulp of Prinsepia utilis. The ripened fruits and soil samples were collected for detailed investigation. The fruits (pulp) were extracted in methanol for analyzing total phenolics and antioxidant activity using a spectrophotometric method and phenolic compounds by high-performance liquid chromatography (HPLC). Similarly, standard methods for soil analysis were used. Results reveal that altitude negatively correlated with total phenolics and flavonoid contents determined by a spectrophotometric method and with specific phenolic compounds determined by HPLC. For instance, Dwarahat population (PU-1) at lower altitude (1400 m asl) exhibited higher values of total phenolics and flavonoids, while total tannin was higher at Nainital (PU-6) population (2000 m asl). The antioxidant activity measured by 2, 2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was found higher in Narayan Ashram (PU-9) population (2750 m asl), while ferric reducing antioxidant power (FRAP) was significantly higher in Badechena (PU-2) population (1600 m asl). Soil nutrients (edaphic factors) showed a good correlation among measured parameters. Neighbor-joining and principal component analysis identified phenolics and antioxidant-rich populations that can be utilized for collection of fruits from these populations.