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Updated: Oct 4, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
[Ecological aspects of postmortem microbiome and possibility of their use in forensic practice]
N A Sidorova1, I A Tolmachev2, O S Lavrukova1
1Petrozavodsk State University, Petrozavodsk, Russia.
Abstract:
Through experimental reproduction of the decomposition process using the cadaver of rabbit Oryctolagus sp., the dynamics of cadaver microflora was studied; the dominant bacterial taxa were isolated in pure culture and identified; their ecological and trophic profiles and biodiversity were described based on the values of Simpson and Menchinic ecological indices. The dependence of cadaver rotting and skeletonization rate on the taxonomic profile of microorganisms, decomposition period, and abiotic environmental factors (temperature, acidity, soil moisture) was demonstrated. The data obtained contribute to justifying the use of microbiological methods in forensic practice. To establish objective causality patterns of microbial transformation of organic matter in nature, further targeted study of ecological patterns of cadaveric microflora is necessary.
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