Complete Genome Sequences of Four Isolated Bacteria from an Undergraduate Microbiology Course Using a Hybrid Assembly
Ciara I Sanders1, Christopher J Ne Ville1, Paul M Orwin1
1Department of Biology, California State University, San Bernardino, San Bernardino, California, USA.
Microbiology Resource Announcements
|February 10, 2022
Summary
Researchers sequenced the genomes of four antibiotic-resistant bacteria isolated during an undergraduate microbiology course. This study reports the complete genomic data for Acinetobacter johnsonii, Aeromonas hydrophila, Bacillus velezensis, and Comamonas thiooxydans.
Area of Science:
- Microbiology
- Genomics
- Bacterial Genetics
Background:
- Antibiotic resistance is a growing global health concern.
- Environmental bacteria can harbor resistance genes.
- Undergraduate courses offer opportunities for authentic research experiences.
Purpose of the Study:
- To isolate and characterize antibiotic-resistant bacteria from environmental samples.
- To perform whole-genome sequencing and assembly of bacterial isolates.
- To report the genomic sequences of four distinct bacterial species.
Main Methods:
- Isolation of bacteria from environmental samples based on antibiotic resistance.
- DNA purification and extraction from bacterial isolates.
- Hybrid sequencing and assembly approach for whole-genome analysis.
Main Results:
- Successfully isolated three Gram-negative and one Gram-positive bacterium.
- Generated and assembled high-quality draft genomes for the isolates.
- Identified the genomes of Acinetobacter johnsonii CSUSB1, Aeromonas hydrophila CSUSB2, Bacillus velezensis CSUSB3, and Comamonas thiooxydans CSUSB4.
Conclusions:
- The study provides valuable genomic resources for four antibiotic-resistant bacterial species.
- This work demonstrates the feasibility of incorporating genome sequencing into undergraduate microbiology curricula.
- The reported genomes can facilitate further research into antibiotic resistance mechanisms and bacterial evolution.
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