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The synthesis and use of oligodeoxynucleotides in plasmid DNA sequencing
The International Journal of Biochemistry
|January 1, 1986
Abstract:
A convenient procedure for the synthesis and purification of oligonucleotides is described. 16-base long primers synthesised by this method were used to investigate DNA sequencing using plasmid DNA as a template. This allowed the further analysis of the E. coli glt A sequence coding for citrate synthase and enabled determination of the 5'-non-coding regulatory region of the aminoglycoside phosphotransferase gene.