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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
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On the road toward highly efficient and large volume three-dimensional-printed liquid chromatography columns?
Fabrice Gritti1, Suhas Nawada2
1Instrument/Core Research/Fundamentals, Waters Corporation, Milford, Massachusetts, USA.
Journal of Separation Science
|February 10, 2022
Summary
Researchers developed 3D-printed liquid chromatography columns with ordered structures, achieving near-unity reduced plate heights. This advance overcomes limitations of traditional packed columns for enhanced separation efficiency.
Area of Science:
- Analytical Chemistry
- Materials Science
- Chemical Engineering
Background:
- Current liquid chromatography (LC) columns suffer from limited performance due to random packing and structural heterogeneity.
- Theoretical efficiency for ideal sphere packings suggests significantly lower plate heights than currently achieved.
Purpose of the Study:
- To develop a novel method for fabricating highly efficient liquid chromatography columns.
- To overcome the limitations of conventional packed columns through ordered structures.
Main Methods:
- 3D printing of a wide inner diameter column using stereolithography of poly(ethylene glycol diacrylate) resin.
- Integration of photolithography with photomasks to achieve feature sizes below 100 μm.
- Layer-by-layer polymerization using alternating horizontal and vertical pattern masks.
Main Results:
- Achieved minimum reduced plate heights around unity for nonretained analytes, a significant improvement over conventional columns.
- Demonstrated the feasibility of 3D printing ordered macroporous structures for LC applications.
- Identified printing imperfections inherent in the process.
Conclusions:
- 3D-printed ordered structures offer a promising route to overcome the efficiency limitations of conventional packed LC columns.
- Future work should focus on reducing feature size, improving dimensional uniformity, and exploring materials like silica aerogels for enhanced performance.
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