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Related Experiment Video

Updated: Oct 4, 2025

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
06:10

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

Published on: August 15, 2025

714

A Simple Double-Spin Closed Method for Preparing Platelet-Rich Plasma.

Edilson S Machado1,2, Fabiano P Soares1, Roberta S Yamaguchi3

  • 1Pain Medical Center, Regenerar, Porto Alegre, BRA.

Cureus
|February 11, 2022
PubMed
Summary
This summary is machine-generated.

This study presents a new, safe, and reproducible double-spin technique for preparing platelet-rich plasma (PRP) for clinical use. The method effectively concentrates platelets while altering white blood cell counts, offering a valuable tool for medical applications.

Keywords:
double-spingrowth factorsorthobiologicsorthopedicsplatelet-rich plasmaplateletsregenerative medicine

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Area of Science:

  • Biomedical Science
  • Hematology
  • Regenerative Medicine

Background:

  • Platelet-rich plasma (PRP) is utilized in clinical practice for its regenerative properties.
  • Existing methods for PRP extraction vary, necessitating standardized and efficient protocols.

Purpose of the Study:

  • To introduce and analyze a novel double-spin protocol for platelet-rich plasma (PRP) extraction.
  • To compare the efficacy of this new technique against previously described PRP preparation methods.

Main Methods:

  • Sixteen blood samples from healthy volunteers were processed using a new double-spin centrifugation technique without opening the collection container.
  • Descriptive analysis and statistical comparisons of cell and platelet counts were performed between baseline whole blood and the prepared PRP samples.

Main Results:

  • The double-spin technique achieved a mean platelet concentration ratio of 3.47, indicating significant platelet enrichment.
  • A positive correlation was observed between baseline and PRP platelet counts (r=0.56, P=0.023).
  • PRP exhibited increased lymphocyte and monocyte counts but decreased neutrophil and eosinophil counts compared to baseline.

Conclusions:

  • The developed double-spin method provides a safe and easily reproducible means of obtaining platelet-rich plasma.
  • This protocol is suitable for routine clinical application in daily practice.