Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fibronectin potentiates actin polymerization in thrombin-activated platelets.

C S Cierniewski, J Karczewski, M A Kowalska

    Journal of Cellular Biochemistry
    |January 1, 1986
    PubMed
    Summary

    Fibronectin binding to platelet receptors promotes actin polymerization, increasing F-actin incorporation into the cytoskeleton. This suggests fibronectin facilitates cytoskeletal organization in platelets.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Nano Tin/Tin Oxide Attached onto Graphene Oxide Skeleton as a Fluorine Free Anode Material for Lithium-Ion Batteries.

    Inorganic chemistry·2020
    Same author

    Erythrocyte transketolase deficiency in patients suffering from Crohn's disease.

    European review for medical and pharmacological sciences·2019
    Same author

    A modified weighted mixture model for the interpretation of spatial and temporal changes in the microbial communities in drinking water reservoirs using compositional phospholipid fatty acid data.

    Talanta·2016
    Same author

    Intramedullary megakaryocytes internalize released platelet factor 4 and store it in alpha granules.

    Journal of thrombosis and haemostasis : JTH·2015
    Same author

    Fetal membranes as a source of stem cells.

    Advances in medical sciences·2013
    Same author

    Urologic de novo malignancies after kidney transplantation: a single center experience.

    Transplantation proceedings·2012

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Platelet Physiology

    Background:

    • Actin polymerization is crucial for cellular structure and function.
    • Platelets play a key role in hemostasis and thrombosis.
    • Fibronectin is an extracellular matrix protein involved in cell adhesion and signaling.

    Purpose of the Study:

    • To investigate the effect of fibronectin on actin polymerization in platelets.
    • To understand the role of fibronectin-membrane receptor interactions in regulating platelet cytoskeleton.

    Main Methods:

    • Preparation of Triton X-100-insoluble cytoskeleton from thrombin-activated platelets.
    • Monitoring G-actin to F-actin conversion using DNase I inhibition assay.
    • Comparing the effects of fibronectin, fibrinogen, and bovine serum albumin.

    Related Experiment Videos

    Main Results:

    • Fibronectin binding to platelet receptors significantly decreased the level of platelet G-actin.
    • This indicates increased F-actin incorporation into the Triton X-100-insoluble cytoskeleton in the presence of fibronectin.
    • Fibrinogen had a minor effect, and bovine serum albumin showed slight inhibition of actin polymerization.

    Conclusions:

    • Fibronectin facilitates actin polymerization into the platelet cytoskeleton.
    • This process involves interaction with the platelet actomyosin fibrillar system.
    • Fibronectin plays a regulatory role in platelet cytoskeletal dynamics.