Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human IgG subclass assays using a novel assay method.

J Mayus, K Macke, P Shackelford

    Journal of Immunological Methods
    |April 3, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Distinct roles of lymphotoxin alpha and the type I tumor necrosis factor (TNF) receptor in the establishment of follicular dendritic cells from non-bone marrow-derived cells.

    The Journal of experimental medicine·1998
    Same author

    Ca2+-channel-dependent and -independent inhibition of exocytosis by extracellular ATP in voltage-clamped rat adrenal chromaffin cells.

    Pflugers Archiv : European journal of physiology·1998
    Same author

    Protein disulfide isomerase as a regulator of chloroplast translational activation.

    Science (New York, N.Y.)·1998
    Same author

    Amplification and sequencing of end fragments from bacterial artificial chromosome clones by single-primer polymerase chain reaction.

    Analytical biochemistry·1997
    Same author

    Phenobarbital alters protein binding to the CYP2B1/2 phenobarbital-responsive unit in native chromatin.

    The Journal of biological chemistry·1997
    Same author

    Brucella abortus arginase and ornithine cyclodeaminase genes are similar to Ti plasmid arginase and ornithine cyclodeaminase.

    Biochimica et biophysica acta·1997

    This study adapted a particle concentration fluorescence immunoassay for human immunoglobulin G (IgG) subclasses, offering improved sensitivity and speed. However, a monoclonal antibody for the IgG4b epitope showed limited utility in identifying IgG2 deficiency.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Human immunoglobulin G (IgG) subclass assays are crucial for diagnosing immunodeficiencies.
    • Existing assay methods have limitations in sensitivity and throughput.
    • Novel immunoassay adaptation is needed for efficient IgG subclass analysis.

    Purpose of the Study:

    • To adapt a particle concentration fluorescence immunoassay (PCFIA) for human IgG subclass quantification.
    • To evaluate the suitability of polyclonal and monoclonal antibodies for IgG subclass determination using PCFIA.
    • To assess the potential of a monoclonal antibody targeting the IgG4b epitope for screening IgG2 deficiency.

    Main Methods:

    • Adaptation of the particle concentration fluorescence immunoassay (PCFIA) method.
    • Utilized polyclonal and monoclonal antibodies against human IgG subclasses (IgG1, IgG2, IgG3, IgG4).

    Related Experiment Videos

  • Evaluated antibody performance in PCFIA and competitive radioimmunoassay.

    • Main Results:

    • The PCFIA method demonstrated high sensitivity (0.3-3 µg/ml) and rapid, automated measurement (2 hours).
    • Successful adaptation of antibodies for IgG1, IgG2, IgG3, and the IgG4b epitope was achieved.
    • No suitable monoclonal antibody for IgG4 was identified for the PCFIA method.
    • A monoclonal antibody for IgG4b showed potential for screening IgG2 deficiency but identified only half of deficient patients.

    Conclusions:

    • The adapted PCFIA offers a sensitive and efficient platform for human IgG subclass analysis.
    • Monoclonal antibodies are valuable reagents for specific IgG subclass detection.
    • The IgG4b-targeting antibody has limited diagnostic value for IgG2 deficiency screening compared to specific IgG2 antibodies.