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A high-resolution melting assay to differentiate a peste des petits ruminants virus vaccine strain from field
Mustafa Emin Oz1, Emrah Torlak2
1Molecular Microbiology Laboratory, Veterinary Control Institute, 42090, Konya, Turkey. oz.mustafaemin@gmail.com.
Abstract:
Peste des petits ruminants (PPR) mostly affects small ruminants. Vaccination with attenuated vaccines derived from PPR virus (PPRV) provides successful protection against the disease. However, current molecular methods are unable to distinguish vaccine strains from field strains. In this study, we used an EvaGreen-based high-resolution melting (HRM) assay to differentiate a PPRV vaccine strain (Nigeria 75/1) from Turkish field isolates of lineage IV based on melting peaks and melting profiles.
