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Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
Published on: July 12, 2018
Identifying Bacterial Lineages in Salmonella by Flow Cytometry
Rocío Fernández-Fernández1, David R Olivenza1, María Antonia Sánchez-Romero2
1Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Seville, Spain.
Abstract:
Advances in technologies that permit high-resolution analysis of events in single cells have revealed that phenotypic heterogeneity is a widespread phenomenon in bacteria. Flow cytometry has the potential to describe the distribution of cellular properties within a population of bacterial cells and has yielded invaluable information about the ability of isogenic cells to diversify into phenotypic subpopulations. This review will discuss several single-cell approaches that have recently been applied to define phenotypic heterogeneity in populations of Salmonella enterica.
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